Separation of Proteins by Thin-Layer Gel Filtration.

Refolding proteins by gel filtration chromatography.

We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique. We have extended this strategy to th...

Separation of antigens of Streptococcus faecalis by gel filtration.

Separation of soluble Brucella antigens by gel-filtration chromatography.

Soluble precipitating antigens of Brucella suis have been, in various degrees, purified by filtration on Sephadex gels. The most useful gels employed were Sephadex G-150, Sephadex G-200, and Sepharose 4B. Although not all fractions proved to be immunologically pure, some crude molecular-size estimates of most of the 13 soluble antigens of the Brucella cell could be given. In addition, monospeci...

Thin-layer acrylamide gel electrophoresis.

A thin-layer acrylamide gel electrophoresis technique suitable for analysis of serum or urine proteins is described in detail. The method gives sharp differentiation of protein fractions and is particularly suitable for the analysis of biological solutions containing proteins in low concentration.

Analysis of urinary proteins in urolithiasis by isoelectric focusing using ultra-thin-layer gel.

greater in the mutant ( P < 0.00 1 ) and remained constant with age. The heart was not affected. These results indicate that the myotonic mutation has a signficant effect on the total glucose-6-phosphatase activities of skeletal muscle and kidney, but whereas the former is raised the latter is lowered. There is a small effect on the liver and no effect on the heart. Myotonic dystrophy is known ...